Description
Description: This assay employs a two-site sandwich ELISA to quantitate GDI2 in samples. An antibody specific for GDI2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GDI2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GDI2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GDI2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: GDP dissociation inhibitors are proteins that regulate the GDP-GTP exchange reaction of members of the rab family, small GTP-binding proteins of the ras superfamily, that are involved in vesicular trafficking of molecules between cellular organelles. GDIs slow the rate of dissociation of GDP from rab proteins and release GDP from membrane-bound rabs. GDI2 is ubiquitously expressed. The GDI2 gene contains many repetitive elements indicating that it may be prone to inversion/deletion rearrangements.
Bachner et al. (1995) studied expression patterns of the 2 human genes. They showed that the 2.5-kb mRNA for RABGDIB is ubiquitously expressed, in contrast to RABGDIA, which is expressed primarily in neural and sensory tissues